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Our Customer Service team is available from 7:30am to 8pm, ET, Monday through Friday. Live chat is available from 8am to 6pm ET, Monday-Friday.
We serve educators in more than 170 countries worldwide. Create a quote request on our website or contact our International Sales Team.
Login or register now to maximize your savings and access profile information, order history, tracking, shopping lists, and more.
Intermediate—Easy to perform; requires some background knowledge.
Using a fluorescent dye, students investigate the conditions that influence DNA structure and its transition from double helix to single strand, and vice versa. They carry out 3 investigations exploring DNA composition, concentration, the chemical milieu, and their influence on Watson-Crick pairing.
Intermediate—Easy to perform; requires some background knowledge.
In this lab, students use a fluorescent dye to investigate the conditions that influence DNA structure and its transition from double helix to single strand, and vice versa. Students can test conditions that influence Watson-Crick base pairing between complementary DNA strands, using temperature to differentiate between DNA of different sequences, and establishing the melting temperature of each sample. They carry out 3 investigations exploring DNA composition, concentration, the chemical milieu, and their influence on Watson-Crick pairing. Kit includes sufficient materials for a class of 32 working in groups. Note: Ships with perishable materials. Keep refrigerated until use.
Components | Qty | Included? |
---|---|---|
DNA Sample | 1 | Included |
DNA Binding Dye | 1 | Included |
Glow Lab Dilution Buffer | 1 | Included |
Microtubes | 24 | Included |
Digital Student Guide (download from miniPCR website) | 1 | Needed, Not Included |
Digital Instructor's Guide (download from miniPCR website) | 1 | Needed, Not Included |
Thermal Cycler | 1 | Needed, Not Included |
Blue Light Transilluminator | 1 | Needed, Not Included |
Micropipets (2 to 20 µL and 20 to 200 µL) | 12 | Needed, Not Included |