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Find your Carolina Sales Manager for K-8 Smithsonian and Building Blocks of Science Programs.
We serve educators in more than 170 countries worldwide. Create a quote request on our website or contact our International Sales Team.
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Intermediate—Easy to perform; requires some background knowledge.
Using a fluorescent dye, students investigate the conditions that influence DNA structure and its transition from double helix to single strand, and vice versa. They carry out 3 investigations exploring DNA composition, concentration, the chemical milieu, and their influence on Watson-Crick pairing.
Intermediate—Easy to perform; requires some background knowledge.
In this lab, students use a fluorescent dye to investigate the conditions that influence DNA structure and its transition from double helix to single strand, and vice versa. Students can test conditions that influence Watson-Crick base pairing between complementary DNA strands, using temperature to differentiate between DNA of different sequences, and establishing the melting temperature of each sample. They carry out 3 investigations exploring DNA composition, concentration, the chemical milieu, and their influence on Watson-Crick pairing. Kit includes sufficient materials for a class of 32 working in groups. Note: Ships with perishable materials. Keep refrigerated until use.
This item is only available at Carolina.com.| Components | Qty | Included? |
|---|---|---|
| DNA Sample | 1 | Included |
| DNA Binding Dye | 1 | Included |
| Glow Lab Dilution Buffer | 1 | Included |
| Microtubes | 24 | Included |
| Digital Student Guide (download from miniPCR website) | 1 | Needed, Not Included |
| Digital Instructor's Guide (download from miniPCR website) | 1 | Needed, Not Included |
| Thermal Cycler | 1 | Needed, Not Included |
| Blue Light Transilluminator | 1 | Needed, Not Included |
| Micropipets (2 to 20 µL and 20 to 200 µL) | 12 | Needed, Not Included |
