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Grades 9–12. Students investigate possible water source contamination by coliform bacteria. Using sterile technique, they collect water samples and plate them on selective media to differentiate between general coliform, Escherichia coli, and noncoliform bacteria colonies. After 24 to 48 hr, results are analyzed. In the second investigation, students analyze samples and propose design solutions to reduce the environmental impacts of human activities linked to bacterial contamination of water.
After collecting various water samples, use sterile technique to identify and differentiate Escherichia coli from other coliforms, then propose design solutions that reduce the environmental impact of human activities.
Related PE: HS-LS4-6
Time Requirement: 2 45-minute Class Periods
SEP: Constructing Explanations and Designing Solutions
CCC: Cause and Effect
DCI: LS2.C: Ecosystem Dynamics, Fuctioning, and Resilience; ETS1.B:Developing Possible Solutions
Grades 9–12. Students investigate possible water source contamination by coliform bacteria. Using sterile technique, they collect local water samples from approved locations and then plate them on selective media. The media allows students to differentiate between general coliform, Escherichia coli, and noncoliform bacteria colonies based on color. Students analyze the colonies 24 to 48 hr after plating. Based on the number of coliform and E. coli cultures, as well as any dilution factor, students determine if their samples meet standards for different water uses in their area.
In the second inquiry-based investigation, students analyze water samples from a fictional municipality for the presence of coliform and E. coli cultures. Then, based on activity results and inferred sources of possible contamination, students propose design solutions to reduce the environmental impacts of human activities linked to the water’s bacterial contamination. This kit helps address Next Generation Science Standard* HS-LS2-7, includes 1-year access to digital resources, and accommodates up to 10 groups of students.
Note: Kit includes a prepaid coupon to request perishable materials later at your convenience. Contact us or return the coupon to request delivery of perishables.
*Next Generation Science Standards® is a registered trademark of Achieve. Neither Achieve nor the lead states and partners that developed the Next Generation Science Standards were involved in the production of this product, and do not endorse it.
|Escherichia coli B MicroKwik Culture®||1||Included|
|Enterobacter aerogenes MicroKwik Culture®||1||Included|
|Dehydrated Chromogenic Medium, 5.2 g||1||Included|
|Sterile Collection Tubes||30||Included|
|Sterile Bulb Pipets||60||Included|
|Sterile Petri Dishes, 60 x 15 mm||20||Included|
|Carolina Science Online® Resources (1-year subscription)||1||Included|
|Teacher's Manual with Reproducible Student Guide||1||Included|
|Permanent Markers||10||Needed, Not Included|
|Small Resealable Plastic Bags (at least 7" W)||40||Needed, Not Included|
|Lab Thermometer||1||Needed, Not Included|
|Bleach||Needed, Not Included|
|Ethanol or Isopropanol||Needed, Not Included|
|Water Samples (collected by students)||Needed, Not Included|
|Sterile Distilled Water||Needed, Not Included|
|Tubes for Dilution (if necessary)||Needed, Not Included|
|Parafilm®||Needed, Not Included|
|Paper Towels||Needed, Not Included|
|Soap||Needed, Not Included|
|Erlenmeyer Flask, 250 mL||Needed, Not Included|
|Hot Plate||Needed, Not Included|
|Incubator (optional)||Needed, Not Included|