Exploring Electrophoresis Using Forensic DNA Evidence Kit

Use evidence found in DNA fingerprint patterns to argue which suspect committed a crime.

PE: HS-LS3-2
Time Requirement: 2 45-minute Class Periods

SEP: Engaging in argument from evidence
CCC: Cause and Effect
DCI: LS3.B: Variation of Traits

Intermediate—Easy to perform; requires some background knowledge.

• Introduces students to the use of DNA analysis in forensic investigations
• Perform a technically simple, hands-on experiment in 45 minutes with 9-V batteries
• Discuss advanced topics of PCR and DNA fingerprinting

Solve a forensic mystery using the tools of biotechnology and the concept of DNA fingerprinting. Students cast agarose gels, load predigested DNA samples, and perform electrophoresis with one to three 9-V batteries. They stain the gels and analyze the banding patterns of DNA collected from the suspects and the crime scene. Includes beautifully illustrated instructions, free 1-year access to digital resources that support NGSS instruction, as well as needed materials and equipment to perform the lab twice. Materials are durable and reusable with replacement parts available.