Advanced—For experienced high school and college classes; requires some technical skill.
Making a genotype-to-phenotype connection using Wisconsin Fast Plants®
This lab explores the connection between Mendelian genetics and molecular biology through the analysis of the mutation responsible for the non-purple stem trait in Wisconsin Fast Plants®. Students predict the phenotype and genotype for stem color in the F2 generation of a monohybrid cross and propose an explanation as to why the anl allele leads to a non-purple stem phenotype, whereas the ANL allele leads to the purple stem phenotype. Students finally plant F2 seeds on a monohybrid cross that will be used for phenotypic and molecular analysis. This lab also requires students to familiarize themselves with bioinformatics programs in which they practice analyzing sequence data of purple stem and non-purple stem alleles of the anthocyaninless genes. They then translate the sequences and discuss how the mutations found in non-purple stem alleles impact gene function.
Each kit contains instructions and enough materials for 24 students working in groups of 4. The kits also come with free 1-year access to digital resources that support 3-dimensional instruction for NGSS. Note: Order the kit with the perishable materials included or with a prepaid coupon to request perishables later at your convenience. Contact us or return the coupon to request delivery of perishable materials.